Assessor Resource

MSL973020
Perform histological procedures

Assessment tool

Version 1.0
Issue Date: May 2024


This unit of competency describes the skills and knowledge to perform straightforward histological procedures involving processing and sectioning (by hand or rotary microtome) of plant and/or animal tissues in paraffin wax.

This unit of competency applies to laboratory assistants working in biomedical, biotechnology, environmental and education sectors. Personnel will work under direct supervision and have ready access to workplace procedures.

No licensing or certification requirements exist at the time of publication. However, regulations and/or external accreditation requirements for laboratory operations exist, so local requirements should be checked. Relevant legislation, industry standards and codes of practice within Australia must also be applied.

You may want to include more information here about the target group and the purpose of the assessments (eg formative, summative, recognition)

Submission Requirements

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Assessment Tasks

Copy and paste from the following data to produce each assessment task. Write these in plain English and spell out how, when and where the task is to be carried out, under what conditions, and what resources are needed. Include guidelines about how well the candidate has to perform a task for it to be judged satisfactory.
Copy and paste from the following performance criteria to create an observation checklist for each task. When you have finished writing your assessment tool every one of these must have been addressed, preferably several times in a variety of contexts. To ensure this occurs download the assessment matrix for the unit; enter each assessment task as a column header and place check marks against each performance criteria that task addresses.

Observation Checklist

Tasks to be observed according to workplace/college/TAFE policy and procedures, relevant legislation and Codes of Practice Yes No Comments/feedback
Perform pre-use checks to ensure processor is fit for purpose 
Select program and reagents for processing 
Inspect processor reagents for deterioration and adequate volume, and report any items requiring replacement 
Check processor for errors that occurred during processing sequence 
Check that volume of wax is sufficient for uninterrupted embedding of processor load 
Check that temperature of wax is suitable for embedding process 
Embed tissue in correct orientation 
Apply procedures to prevent cross-contamination between tissues 
Inspect blocks, reject and re-embed items that do not meet quality control standards 
Select reagents specified in the method 
Stain sections according to the method 
Examine sections microscopically to ensure expected staining outcomes have been achieved 
Mount sections to ensure long-term preservation 
Attach permanent labels giving specimen details according to workplace traceability requirements 
 
 
Check that volume of wax is sufficient for uninterrupted embedding of processor load 
 
 
 
 
Label required number of microscope slides in accordance with workplace traceability requirements 
 
 
 
 
 
 
Select reagents specified in the method 
 
 
 
 
Ensure personal safety and minimise cross-contamination through the use of personal protective equipment (PPE) 
 

Forms

Assessment Cover Sheet

MSL973020 - Perform histological procedures
Assessment task 1: [title]

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Assessment Record Sheet

MSL973020 - Perform histological procedures

Student name:

Student ID:

Assessment task 1: [title] Result: Competent Not yet competent

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Overall assessment result: Competent Not yet competent

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